Автор: Vadim Sharov
В календаре: Конференции, выставки и семинары
Дата: 31 Окт 2019 (Событие на один день)
Investigation of Endothelial Cell Viability and Growth on 3D printed GelMa Vascular Networks
DATE: October 31, 2019
A major limitation for the development of 3D engineered tissues is the absence of viable and perfusable vasculature [1-3]. As a precursor to vascularized adipose tissue, cylindrical channels were formed in a cast gelatin methacrylate (GelMA) construct by printings sacrificial networks of Pluronic F127. Human umbilical vein endothelial cells (HUVECs) were seeded and cultured within the 3D printed channels, while Adipose derived stem cells (ADSCs) were cultured in the GelMa prior to casting the 3D printed channels.
The hydrogel was characterized by NMR, surface tension, contact angle and DMA. Pluronic filaments were printed onto glass slides using a robotic printer I&J 7300-LF (Fishnar, UK). HUVECs (PromoCell, UK) were cultured on GelMa substrate, whilst ADSCs (ThermoFisher) were embedded within the GelMa. Live/Dead and Alamar Blue assays were used to assess the cells’ viability and proliferation respectively. Phalloidin staining was used to assess actin cytoskeleton organization.