Christopher Chouinard, PhD
Assistant Professor of Chemistry, Department of Biomedical and Chemical Engineering and Sciences (BCES), Florida Institute of Technology
DATE: October 16, 2019
Ion mobility-mass spectrometry (IM/MS) is a rapid, gas-phase separation technique that has become an integral part of the analytical repertoire of techniques for -omics. This method couples well with existing protocols (i.e., LC/MS) by affording an additional dimension of separation, and provides a characteristic measure of an analyte’s size, shape, and charge in the form of a collision cross section (CCS). CCS can be used to increase confidence in quantitative measurement of known compounds, thus reducing interferences and improving S:N ratios, and, also serves as a defining feature in identification of unknowns. Furthermore, this separation based on structure (as opposed to mass-to-charge alone) allows for distinction of structural and stereoisomers of potential biological importance. This presentation will cover the basic operating principles of IMS and current instrumentation; applications to metabolomics, lipidomics, and proteomics; and future directions for IM/MS.
- Understand the basic operating principles of IMS and current instrumentation
- Understand current applications to metabolomics, lipidomics, and proteomics